Hatsune Miku Colorful Stage features a cast of 20 characters with fully voiced Story Chapters. The players will need to collect character cards and tap, hold, and flick to the rhythm. Hatsune Miku Colorful Stage gives you the feature to play on your own or create a room with four friends. You can also customize your band members with a variety of options.
The characters of Hatsune Miku Colorful Stage are categorized into different tiers below. The best characters in tier SS are Kohane Azusawa and Saki Tenma while Tsukasa Tenma and Nene Kusanagi are the popular ones in tier S. Airi Momoi and Shizuku Hinomori rank in the A tier. Some characters in tier B are Meiko and Kaito.
SS Tier
| Characters | Tier |
| Kohane Azusawa | SS |
| Saki Tenma | SS |
| Mizuki Akiyama | SS |
| Emu Otori | SS |
S Tier
| Characters | Tier |
| Tsukasa Tenma | S |
| Nene Kusanagi | S |
| Toya Aoyagi | S |
| Kanade Yoisaki | S |
| Hoshino Ichika | S |
A Tier
| Characters | Tier |
| Airi Momoi | A |
| Shizuku Hinomori | A |
| Minori Hinasato | A |
| Kagamine Rin | A |
B Tier
| Characters | Tier |
| Meiko | B |
| Kaito | B |
| Hatsune Miku | B |
| Ichika Hoshino | B |